OpenCFU, a New Free and Open-Source Software to Count Cell Colonies and Other Circular Objects
An original arbitrary square image was either tiled to itself or scaled-up (A) in order to obtain a range of square images featuring an increasing number of colonies or increasingly large colonies, respectively. The processing time of the three methods for these images was estimated in both cases (B). On the tested range of resolutions, OpenCFU was faster than both NICE and the ImageJ macro (IJM). The segments joining points do not represent data, but only aid readability.