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Single-Cell and Population NF-κB Dynamic Responses Depend on Lipopolysaccharide Preparation

Figure 3

Activation dynamics for each of the LPS preparations and several concentrations and summary statistical comparisons.

(A) As in Figure 1, the average and ten randomly selected traces of active cells are shown, as well as Time to Peak, Peak Amp and N. See Figure 1 legend for more details. Concentrations are indicated at left, and the preparation at top. The very lowest and highest concentrations are not shown here (but appear in Figure 4), because virtually no cells were found to be active in the first case, and the traces are essentially identical to their nearest neighbor in the second. (B) Time-to-peak values for each LPS preparation are shown with standard deviations, across each concentration. LPS preparations are indicated with different colors as labeled in D. (C) Peak amplitude values for each LPS preparation are shown with standard deviations across each concentration. (D) The correspondence between time-to-peak and peak amplitude is shown for each LPS preparation, including all concentrations.

Figure 3

doi: https://doi.org/10.1371/journal.pone.0053222.g003