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Microfabricated Modular Scale-Down Device for Regenerative Medicine Process Development

Figure 6

Monitoring a hESC colony in the microfabricated culture device during the course of an experiment.

The same colony is shown after (a) 1 day static culture, (b) 1 day perfused culture and (c) 2 days perfused culture. The columns show, from left to right, the raw phase contrast image taken with a 4× objective, an overlay of the automated detection using the image processing algorithm, and the detected area. The scale bars are 500 µm.

Figure 6