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Microfabricated Modular Scale-Down Device for Regenerative Medicine Process Development

Figure 5

Staining of hESC colonies following perfusion culture.

Representative images of the feeder cells and hESC colonies in the culture device after 2 days of continuous perfusion culture. Each row shows the phase contrast images (a, e) of the feeder-attached hESC colonies and the corresponding results from DAPI (b, f,) and pluripotency marker staining for Oct-4 (c), Tra-1-81 (d) and SSEA-3 (g). All images were taken with a 20× objective, scale bar is 200 µm.

Figure 5