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Microfabricated Modular Scale-Down Device for Regenerative Medicine Process Development

Figure 4

Co-cultured hESCs in microfabricated culture device and control dish.

Representative phase contrast images of iMEF feeder cells and individual hESC colonies cultured in the microfabricated culture device (a-c) and in the control dishes (d-f). The same two colonies are shown at each of three time points; after 1 day of static culture (a, d), after 1 day of the perfused culture (b, e), and at the end of the 2 days of perfused culture (c, f). All images were taken with a 4× objective, scale bar is 500 µm.

Figure 4

doi: https://doi.org/10.1371/journal.pone.0052246.g004