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Expression, Characterization, and Cellular Localization of Knowpains, Papain-Like Cysteine Proteases of the Plasmodium knowlesi Malaria Parasite

Figure 4

pH stability of knowpains.

Knowpains were incubated at pH 5.5 (A) or pH 7.5 (B), identical aliquots were taken out in the beginning (T0) and after indicated time intervals and assessed for hydrolysis of Z-LR-AMC as described in the Materials and Methods section. Activities were expressed as % of T0 activity and plotted against time intervals (Time). Data shown are means ± standard error from three independent experiments in triplicate (except for KP3, two experiments in duplicate).

Figure 4

doi: https://doi.org/10.1371/journal.pone.0051619.g004