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High Yield Production and Refolding of the Double-Knot Toxin, an Activator of TRPV1 Channels

Figure 1

DNA construct encoding DkTx.

(A) Design of a synthetic DkTx gene. (B) Sequence of the toxins used in the experiments. Note that DkTx has an N-terminal Gly residue because of the hydroxyl amine cleavage of Asn-Gly sequence added to the N-terminus.

Figure 1

doi: https://doi.org/10.1371/journal.pone.0051516.g001