Five Different Piscidins from Nile Tilapia, Oreochromis niloticus: Analysis of Their Expressions and Biological Functions
(a) A qRT-PCR analysis of Nile tilapia piscidins (TP2, -3, and -4) and ubiquitin expression in various tissues (gills, liver, intestines, skin, head kidneys (abbreviated as ‘kidneys’), and spleen) of Nile tilapia. (b) qRT-PCR analysis of Nile tilapia piscidin transcripts in various tissues (gills, liver, intestines, skin, kidney, and spleen) at two time points (24 and 48 h) after an intraperitoneal injection of Streptococcus agalactiae (SA47) or Vibrio vulnificus (204). Wild-type (WT) indicates no bacteria were injected, and the fish experienced no injection. Expression data are presented as the mean (± standard error; SE) relative quantity normalized to ubiquitin. Relative quantities were calculated to the individual (WT) with the lowest normalized piscidin expression. Data (mean ± SE) with different letters significantly differ (p<0.05) among tissues for single-gene comparisons.