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Spastin's Microtubule-Binding Properties and Comparison to Katanin

Figure 11

Nucleotide-Dependence of the Katanin-Microtubule Interaction.

Panel A shows a SDS-gel of co-sedimentation assays with katanin (E309Q mutant, 1 mM ATP) and a constant concentration of microtubules (2 µM; indicated by a dotted line). With increasing katanin concentrations, an increasing amount of protein is co-sedimented with microtubules. Panel B: Plot of the densitometric analysis as in Figure 10. Panel C shows the same experiment for wild type katanin.

Figure 11

doi: https://doi.org/10.1371/journal.pone.0050161.g011