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Spastin's Microtubule-Binding Properties and Comparison to Katanin

Figure 7

Katanin Interaction with Subtilisin-treated Microtubules.

Panel A shows fluorescence microscopy images of GFP HsKatanin (green) and microtubules. The fluorescent, red parts of microtubules are subtilisin-treated, native stretches are unlabeled. Panel B shows a comparison of binding curves obtained by co-sedimentation of HsKatanin and microtubules. The fraction of bound enzyme was determined by SDS-PAGE of supernatants and pellets. The lines are connecting the data points without a specific model.

Figure 7