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Spastin's Microtubule-Binding Properties and Comparison to Katanin

Figure 3

Diffusion of Katanin along Microtubules.

Panel A shows an image sequence of GFP-FLAG HsKatanin (green) on a microtubule (red). The scale bar is 5 µm, the time between two frames was approximately 1 s (for analysis, the full time resolution was used; see Supporting Movie S2). Panel B shows the distribution of diffusion coefficients of 30 molecules, analyzed as in Figure 2. The distribution of all local diffusion coefficients of all molecules is plotted in the histogram. Panel C plots a histogram of distances from frame to frame with a Gaussian fit.

Figure 3