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Biomarkers of Whale Shark Health: A Metabolomic Approach

Figure 1

Typical 1H NMR spectrum of whale shark serum:

(a) Full spectrum: the signal at 3.27 ppm corresponds to trimethylamine-N-oxide (TMAO). Two other major peaks are due to an internal reference (TMSP; used for referencing the chemical shift scale) and residual protons in the solvent. (b) Spectrum (a) after pre-processing (see Methodology). The grey bars depict spectral regions which were excluded from PCA.

Figure 1

doi: https://doi.org/10.1371/journal.pone.0049379.g001