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Neurotensin and CRH Interactions Augment Human Mast Cell Activation

Figure 5

CRH induces NT and NTR gene expression in human mast cells.

(A) NT and (B) NTR gene expression in LAD2 cells following incubation with the indicated concentrations of CRH for 6 h. Relative mRNA expression was measured by quantitative qPCR, normalized to GAPDH, and expressed relatively to the untreated cells (control). (C) Western blot analysis of NTR following incubation with CRH (10 µM) for 24 h. Tubulin was used as an internal control. For all the experiments, n = 3; *p<0.05, **p<0.01, compared to control.

Figure 5

doi: https://doi.org/10.1371/journal.pone.0048934.g005