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Neurotensin and CRH Interactions Augment Human Mast Cell Activation

Figure 4

NT induces CRHR-1 expression in human mast cells.

(A) LAD2 cells CRHR-1 gene expression was assessed following incubation with different concentrations of NT for 6 h. SP was used as a “positive” control to stimulate mast cell degranulation. Relative mRNA expression was measured by quantitative qPCR, normalized to GAPDH, and expressed relatively to the untreated cells (control). (B) Detection of CRHR-1 in LAD2 cells by Western blot analysis following incubation with NT (10 µM) for 24 or 48 h is shown. For all experiments, n = 3; *p<0.05, **p<0.01, compared to control and (C) CRHR-1 detection by FACS analysis in LAD2 cells following treatment with NT (1, 10 µM) for 48 h; y-axis indicates “counts” of cells, while the x-axis indicates log fluorescence intensity. For all experiments, n = 3; *p<0.05, **p<0.01, compared to control.

Figure 4

doi: https://doi.org/10.1371/journal.pone.0048934.g004