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DNA-Interactive Properties of Crotamine, a Cell-Penetrating Polypeptide and a Potential Drug Carrier

Figure 1

Spectrophotometry of crotamine-DNA mixtures in 0.02 M Hepes, pH 7.5, 0.01 M NaCl, 0.0001 M EDTA (standard buffer with indicated [NaCl]).

A. Spectra of 4.1×10−5 M ds calf thymus DNA +4.3×10−6 M crotamine. ––––––, DNA alone; · · · · ·, crotamine alone; – – – –, DNA + crotamine prior to centrifuging; – · – · – · –, DNA + crotamine supernatant after centrifuging; – · · – · · – · · –, sum of individual DNA and crotamine spectra. B. Spectra of 2.5×10−5 M(p) 25-mer (CCG)8C and 2.5×10−6 M crotamine. ––––––, DNA alone; · · · · ·, crotamine alone; – – – –, DNA + crotamine. C. Titration of ds calf thymus DNA with crotamine; starting [DNA] = 2.22×10−5 M(p). D. Titration of d(CCG)8C with crotamine; starting [DNA] = 1.21×10−4 M(p). E. Titration of d(CCG)8C•G(CGG)8 with crotamine; starting [DNA] = 6.2×10−5 M(p).

Figure 1

doi: https://doi.org/10.1371/journal.pone.0048913.g001