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Urotensin II in Invertebrates: From Structure to Function in Aplysia californica

Figure 4

Immunostaining of apUII-positive neurons and fibers in the Aplysia CNS.

(A) The distribution of immunostained neurons in the buccal ganglion is similar to the in-situ hybridization (ISH) staining distribution shown in Figure 2B. Abbreviations, rBG: rostral surface of the buccal ganglion; cBG: caudal surface of the buccal ganglion. Arrowheads point to individual stained neurons. (B) The distribution of immunostained neurons in the cerebral ganglion is similar to the ISH staining distribution shown in Figure 2B. Abbreviations, dCG: dorsal surface of the cerebral ganglion; vCG: ventral surface of the cerebral ganglion; CPC cerebral-pedal connective; A, C, F, neuronal clusters. (C) The distribution of immunostained neurons in the pedal, pleural and abdominal ganglia. Abbreviations, rdPPG: right dorsal pleural-pedal ganglia; ldPPG: left dorsal pleural-pedal ganglia (for these two images, the pleural ganglia are on the left); rvPPG: right ventral pleural-pedal ganglia; lvPPG: left ventral pleural-pedal ganglia (for these two images, the pleural ganglia are on the right); dAG: dorsal abdominal ganglion; vAG: ventral abdominal ganglion. Arrowheads point to stained neurons. Calibration bar for all panels : 200 µm.

Figure 4

doi: https://doi.org/10.1371/journal.pone.0048764.g004