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Urotensin II in Invertebrates: From Structure to Function in Aplysia californica

Figure 3

Mass spectrometric analysis of apUII prohormone processing and structure verification.

(A) Representative MALDI MS spectrum of individual cultured buccal sensory neurons expressing FMRFa, FRFa and apUII prohormones. apUII-derived peptides (in bold), FMRFa, FRFa peptides (a–e) and an apUII linker peptide are labeled. The inset table specifies the accuracy of the apUII peptides measurement; (M+H) is the monoisotopic mass of the protonated molecular ions, (*) denotes the theoretical mass corrected for the difference from the disulfide bond. (B) Fragmentation spectrum of apUII peptide S [93]-S [112] detected in a +2 charge state with the two red Cys residues highlighted (lower case c). (C) Fragmentation spectrum of apUII peptide S [45]-Q [82] detected in a +2 charge state.

Figure 3

doi: https://doi.org/10.1371/journal.pone.0048764.g003