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Immunization with a Hemagglutinin-Derived Synthetic Peptide Formulated with a CpG-DNA-Liposome Complex Induced Protection against Lethal Influenza Virus Infection in Mice

Figure 9

Prophylactic efficacy of a complex of hH1N1-HK HA233 and Lipoplex(O) against maA/WSN/1933 virus.

BALB/c mice were immunized i.p. two times with a complex of MB-ODN 4531(O) encapsulated in DOPE:CHEMS (Lipoplex(O)), a complex of hH1N1-HK HA233 encapsulated in DOPE:CHEMS (DOPE:CHEMS+hH1N1-HK HA233), or a complex of hH1N1-HK HA233 and Lipoplex(O) (Lipoplex(O)+hH1N1-HK HA233). The immunized mice were challenged intranasally with the maA/WSN/1933 virus. After the virus challenge, the survival rate (A) and the body weight (B) were recorded for 21 days (N = 8/group). Lungs were collected at 3 days or 6 days after the challenge with the maA/WSN/33 virus (N = 3/group) (C). Scale bars in (C), 100 µm. The lung viral titers were measured by means of a plaque assay to estimate the viral clearance at 3 days or 6 days after the challenge with the maA/WSN/1933 virus (D).

Figure 9

doi: https://doi.org/10.1371/journal.pone.0048750.g009