Advertisement
Browse Subject Areas
?

Click through the PLOS taxonomy to find articles in your field.

For more information about PLOS Subject Areas, click here.

< Back to Article

Immunization with a Hemagglutinin-Derived Synthetic Peptide Formulated with a CpG-DNA-Liposome Complex Induced Protection against Lethal Influenza Virus Infection in Mice

Figure 3

Effect of liposome composition, CG dinucleotide and backbone modification on antibody production.

(A) Effect of the liposome composition. Three BALB/c mice were injected i.p. with a complex of hH5N1 HA233 and MB-ODN 4531(O) co-encapsulated in indicated liposomes on three occasions. The antisera were collected, and then amounts of hH5N1 HA233 epitope-specific total IgG were measured by ELISA. (B) Effect of CG dinucleotide and phosphorothioate backbone modification. Three BALB/c mice were injected i.p. with hH5N1 HA233 and DOPE:CHEMS (1∶1 ratio)-co-encapsulated MB-ODN 4531(O) (Lipoplex(O)+hH5N1 HA233), MB-ODN 4531GC(O) (LipoplexGC(O)+hH5N1 HA233), MB-ODN 4531(S) (Lipoplex(S)+hH5N1 HA233), and complementary MB-ODN 4531(S) (Lipoplex 4531(S)CS+hH5N1 HA233) on three occasions. The antisera were collected, and amounts of hH5N1 HA233-specific total IgG were measured by ELISA.

Figure 3

doi: https://doi.org/10.1371/journal.pone.0048750.g003