The Structural Dynamics of the Flavivirus Fusion Peptide–Membrane Interaction
(A) The fluorescence intensity emission spectra of FLAG in the absence (solid line) or presence of SDS (dashed line) and n-OGP micelles (dotted line) at pH 7.4. (B–E) Stern-Volmer plots of fluorescence quenching of FLAG and FLAH by acrylamide at 37°C in micelle-free buffer (triangles) or in the presence of different micelles (squares). Stern-Volmer plots for FLAG (B and D) and FLAH (C and E) in the presence of 10 mM SDS (B and C) and 40 mM n-OGP (D and E). The pH 7.4 data are shown as closed symbols, and the pH 5.5 data as open symbols. FP fluorescence was recorded at 349 nm (micelle-free buffer), 334 nm (in the presence of SDS micelles), and 346 nm (in the presence of n-OGP micelles).