Determining Biophysical Protein Stability in Lysates by a Fast Proteolysis Assay, FASTpp
Figure 2
Thermolysin is active from 20°C to 80°C.
Thermal dependence of intrinsic rates of proteolysis of TL from 20°C to 80°C. After extensive thermal pre-equilibration, we determined kinetic traces of cleavage of an unstructured model substrate of TL, in triplicate for each temperature. The obtained rates were extrapolated based on empirically derived formulas to final concentrations of 0.1 g/L and on top 0.01 g/L of TL [1].