In-Depth Investigation of Archival and Prospectively Collected Samples Reveals No Evidence for XMRV Infection in Prostate Cancer
RNA extracts of 22Rv1 and LNCaP cells, prostate cancer tissues from the 2006 Urisman, et al. study [VP35, VP42, and VP62(2006)], and re-extracted tissue from the VP62 sample, VP62(2012), were analyzed by unbiased deep sequencing. Reads are mapped to the previously sequenced XMRV genome corresponding to each of the samples, with the exception of reads from LNCaP, which are mapped to the 22Rv1-associated genome (Genbank accession number FN692043). The coverage (y-axis) achieved at each position along the ∼8.2 kB XMRV genome (x-axis) is plotted on a logarithmic scale. Abbreviations: nt, nucleotide.