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Targeted DNA Methylation Using an Artificially Bisected M.HhaI Fused to Zinc Fingers

Figure 1

Schematic depictions of sequences and nomenclature of modeled protein/DNA complexes.

(A) Sequences of zinc fingers fused to fragments of M.HhaI methyltransferase. Numbers in brackets correspond to the amino acid numbers. Black segments correspond to linker sequences. (B) The orientation of the zinc finger binding sites relative to the intended methylation target site (the circle). The orientations depicted are the ones that would position the indicated protein pairs over the targeted CpG site. (C) Molecular model of a particular NZ/CZ construct containing the indicated linkers. The base to be methylated is indicated in purple. Models of other complexes can be found in Figure S1.

Figure 1

doi: https://doi.org/10.1371/journal.pone.0044852.g001