Meal Time Shift Disturbs Circadian Rhythmicity along with Metabolic and Behavioral Alterations in Mice
Figure 6
Daily and circadian expressions of LDLR and some LDLR regulatory factors in the mouse liver.
To determine daily expression patterns, young adult C57BL/6J male mice were entrained to a 12L:12D cycle for two weeks and liver samples were quickly obtained at the indicated ZT. For circadian sampling, mice were entrained to a 12L:12D cycle for two weeks and released to constant darkness (DD). On the second day after light-off, liver samples were obtained at the indicated circadian time (CT). RNA isolation, reverse transcription, and real-time polymerase chain reaction to measure specific messages for mouse ldlr, and LDLR regulatory factors. All mRNA levels were normalized to tbp mRNA levels. Data are expressed as mean ± S.E.M. (n = 8).