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Measuring Cation Dependent DNA Polymerase Fidelity Landscapes by Deep Sequencing

Figure 4

Statistical analysis of misincorporation by Dpo4.

(A) Spatial dependence (un-normalized) of Dpo4 error rate at 800 µM Mn2+ on the original template (blue curve), and generalized linear model fits of this data set with respect to itself (green curve), and with respect to the swapped template data set (red curve). (B) Spatial dependence (un-normalized) of Dpo4 error rate at 800 µM Mn2+ on the swapped template (blue curve), and generalized linear model fits of this data set with respect to itself (green curve), and with respect to the original template data set (red curve). (C) Feature weights for generalized linear model fit to Dpo4 original template data. (D) Feature weights for generalized linear model fit to Dpo4 swapped template data. (E) Information gain per base as a function of template position, for discrimination between high (800 µM) and low (75 µM) Mn2+ by Dpo4. (F) Information gain per base as a function of template position, for discrimination between high (7000 µM) and low (1000 µM) Mg2+ by Dpo4.

Figure 4

doi: https://doi.org/10.1371/journal.pone.0043876.g004