Advertisement
Browse Subject Areas
?

Click through the PLOS taxonomy to find articles in your field.

For more information about PLOS Subject Areas, click here.

< Back to Article

Measuring Cation Dependent DNA Polymerase Fidelity Landscapes by Deep Sequencing

Figure 3

Template position dependence of misincorporation rates.

(A) Template position dependence of Dpo4 misincorporation rates on the original template at varying Mn2+ (left) and Mg2+ concentration (right). (B) Template position dependence of Dpo4 misincorporation rates on the swapped template at varying Mn2+ (left) and Mg2+ concentration (right). (C) Template position dependence of Klenow exo misincorporation rates on the original template at varying Mn2+ (left) and Mg2+ concentration (right). Letters above each data point denote the identity of the template base at that position. Grey shaded areas indicate the noise floor, defined as the maximum over positions of the misincorporation rate (plus SEM) observed in an identical experiment with Pfusion HF DNA polymerase (Figure S1). Red (blue) shaded areas in (A) and (B) correspond to shared sub-sequences between the original and the swapped template.

Figure 3

doi: https://doi.org/10.1371/journal.pone.0043876.g003