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Measuring Cation Dependent DNA Polymerase Fidelity Landscapes by Deep Sequencing

Figure 2

Ion-dependent misincorporation rates of Dpo4 and Klenow exo polymerases.

(A, B, C, D) Mean (top) and template-base-specific (bottom) misincorporation rates as a function of Mn2+ (A, C) and Mg2+ (B, D) concentrations. (E, F, G, H) Normalized distributions of misincorporated dNTPs for each template base. (I, J, K, L) Mean (top) and template-base-specific (bottom) misincorporation rates as a function of Ca2+ concentration at 200 µM background Mn2+ (I, K) and 7000 µM background Mg2+ (J, L) concentrations. Errors are given in Tables S12, and are shown as error bars in the line graphs when they are larger than the data symbol.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0043876.g002