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Fertility and Pregnancy-Associated ß-Cell Proliferation in Mice Deficient in Proglucagon-Derived Peptides

Figure 4

Pregnancy-induced ß-cell adaptations in Gcggfp/gfp mice.

A, Pancreatic insulin content was measured after a 16-h fasting period (n = 5–7). B, ß-Cell mass was calculated as the relative percentage area of ß-cells (defined as the percentage area of pancreatic sections that stained positive for insulin) multiplied by the weight of the pancreas (n = 3). C, The average number of ki-67-positive ß-cells per ß-cells (n = 3). D, The percentage of serotonin-positive ß-cells per islet (n = 3). Open squares: Gcg+/+ and Gcggfp/+; closed squares: Gcggfp/gfp. Values are expressed as means±SEM. *p<0.05, **p<0.01, ***p<0.001. E, Immunohistochemical-autofluorescent analyses for GFP (green), insulin (blue) and Ki 67 (red). Islets in non-pregnant Gcggfp/+ (a), pregnant Gcggfp/gfp (b), non-pregnant Gcggfp/+ (c) and pregnant Gcggfp/gfp (d) pancreas. F, Immunohistochemical-autofluorescent analyses for GFP (green), insulin (blue) and serotonin (magenta). Islets in non-pregnant Gcggfp/+ (a), pregnant Gcggfp/gfp (b), non-pregnant Gcggfp/+ (c) and pregnant Gcggfp/gfp (d) pancreas. Scale bars: 100 µm.

Figure 4

doi: https://doi.org/10.1371/journal.pone.0043745.g004