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Differential Glioma-Associated Tumor Antigen Expression Profiles of Human Glioma Cells Grown in Hypoxia

Figure 2

Glioma cells grown under hypoxic conditions express more EZH2, Whsc2 and YKL-40 at the protein level.

U251 cells grown under either hypoxic or normoxic conditions for 1 week, were harvested, fixed and stained for the intracellular presence of EZH2, Whsc2 and YKL-40. Panel A displays the mean peak channel intensity of 10,000 U251 cells analyzed for intracellular EZH2, Whsc2 and YKL-40, while Panel B shows the data using the D54 cells. The asterisk indicates P<0.01 value.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0042661.g002