Differential Glioma-Associated Tumor Antigen Expression Profiles of Human Glioma Cells Grown in Hypoxia
After one week of culture the hypoxic and normoxic grown cells were harvested, lysed and prepared for qRT-PCR analysis using the glioma tumor antigen panel. The cells grown under hypoxic conditions (1% O2) were directly compared to those simultaneously matched cells grown under normoxic conditions for 1 week. Samples were analyzed 3–6 times depending upon the closeness of the results. Data is represented as fold changes ± standard error of the means. Panel A shows TAPP genes that were up-regulated in both cell lines; the horizontal line represents the cut-off value (1.9-fold increase) that we believe represents a biologically significant value. Panel B shows the genes down-regulated in both glioma cells. The horizontal line in this panel represents a ≥−1.9- fold reduction value which we believe represents a threshold of biological significance. The data for the other TAPPs, which did not show any significant change, and is not presented for the sake of brevity. The single asterisk signifies P<0.05, while double asterisk shows P<0.01 value between the data generated from the normoxic and hypoxic cells.