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Evidence of Melanoma in Wild Marine Fish Populations

Figure 2

Microbial analysis of Plectropomus leopardus samples; a) Bacterial 16S rRNA gene fingerprints (represented on Denaturing Gradient Gel Electrophoresis) of fish mucus (SWB) and tissue samples (TSU), standardised for gel-to-gel comparison using BioNumerics; b) resin embed histological section of a healthy fish, stained with the general DNA stain 4′6-diamidino-2-phenylindole (DAPI); c) histological section of the lesion on a diseased fish stained with DAPI, both showing no bacteria within the dermis suggesting the bacteria present in (a) are localised within the mucus layer not within the tissues.

Scale bars = 10 µm.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0041989.g002