Inhibition of Endoplasmic Reticulum Stress Improves Mouse Embryo Development
Figure 6
Effect of TUDCA on apoptosis in mouse blastocyst.
Apoptosis in blastocysts was evaluated using the TUNEL assay. The original magnification was approximately × 200. A. Images of mouse blastocysts cultured with stress inducers in the presence or absence of TUDCA. The green color indicates DNA fragmentation, and nuclei are stained in red; Scale bar, 20 μm. B. Mean values ± SEM of number of DNA fragments in blastocysts cultured with stress inducers in the presence or absence of TUDCA. Each experiment was repeated three times. (*) indicates a statistically significant difference from control (P<0.05).