An Analysis of the Myocardial Transcriptome in a Mouse Model of Cardiac Dysfunction with Decreased Cholinergic Neurotransmission
Figure 1
qPCR analysis confirms the expression pattern of several genes detected in the microarray analysis.
mRNA expression of tRNA splicing endonuclease 3 (Tsen15; panel a), Growth arrest specific 5 (Gas5; panel b), karyopherin alpha 2 (Kpna2; panel c) and suppressor of cytokine signaling 4 (Socs4; panel d) was increased in mutant mice. mRNA analysis of ribonuclease, RNase A family 4 (Rnase4; panel e) and oxoglutarate-dehydrogenase like (Ogdhl; panel f) confirmed transcriptional downregulation in whole heart RNA from the mutant animals. Data represent the mean ± SEM, with n indicated within bars. *p<0.05 versus wild-type mice.