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Visualizing Escherichia coli Sub-Cellular Structure Using Sparse Deconvolution Spatial Light Interference Tomography

Figure 1

Experimental setup.

The SLIM module is attached to a commercial phase contrast microscope (AxioObserver Z1, Zeiss). The first 4-f system (lenses L1 and L2) expands the field of view to maintain the resolution of the microscope. The polarizer, P is used to align the polarization of the field with the slow axis of the Spatial Light Modulator (SLM). Lens L3 projects the back focal plane of the objective, containing the phase ring, onto the SLM which is used to impart phase shifts of 0, π/2, π and 3π/2 to the un-scattered light relative to the scattered light as shown in the inset. Lens L4 then projects the image plane onto the CCD for measurement. From the 4 intensity measurements a quantitative phase map is reconstructed as shown in the inset.

Figure 1

doi: https://doi.org/10.1371/journal.pone.0039816.g001