5-Methylcytosine and 5-Hydroxymethylcytosine Spatiotemporal Profiles in the Mouse Zygote
Figure 4
Relationship between DNA demethylation and replication.
A) Quantification of the 5MeC paternal/maternal ratio in control (methanol) and aphidicolin treated embryos with representative images (z-projections of 3D-stacks). Images were rotated if necessary to have the maternal PN on the left and the paternal PN on the right. Scale Bar: 10 µm. B) Separate quantification of 5MeC for both pronuclei in control and aphidicolin treated embryos. 4 experiments were performed and a total of 59 (5+17+25+12) control embryos versus 61 (7+18+22+14) aphidicolin treated ones were quantified.