In Situ Identification of Plant-Invasive Bacteria with MALDI-TOF Mass Spectrometry
Figure 1
Typical MALDI mass spectra between m/z 3000 and m/z 13000 of S. fredii strain USDA257 grown on TY for 5 days at 28°C (A) or found inside nitrogen-fixing nodules of G. max (B), M. atropurpureum (C), and V. unguiculata (D) collected respectively 35, 49, and 42 days post-inoculation.
Sample preparation included washing steps to remove leghemoglobin, and suspension of bacterial pellets in 25% formic acid. Positions of biomarkers selected for the USDA257 SSp are showed with dashed lines.