eIF2α Kinases Regulate Development through the BzpR Transcription Factor in Dictyostelium discoideum
Figure 4
RT-PCR determination of relative levels of β-galactosidase mRNA in Ax4 and BS167 transformed with pbzpR-9.
Wild type (Ax4) and eIF2α S51A knockin (BS167) cells were grown in the presence of bacteria, harvested, and plated for development. RNA was isolated from growing cells (0) and at the tipped mound (tm), finger/slug (fs), early culminant (ec), and late culminant (lc) stages. Primers used were bzr-10 and lacZ-4. H7 primers were used in parallel reactions to confirm RNA concentrations. Conditions were optimized to reveal differences in RNA levels of two to ten-fold.