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RAMTaB: Robust Alignment of Multi-Tag Bioimages

Figure 3

Construction of a synthetic data set Isyn using a single tag image Isel with center coordinates (xcenter, ycenter) and uniformly distributed random shifts xn∈[−xmin,+xmax] and yn∈[−ymin,+ymax] in both directions.

Intensity variations such as contrast stretching and Gaussian blurring are also introduced randomly in 50% of the images to mimick the random perturbations in pixel intensities during the image acquisition process (The image here has been inverted for visibility purpose.)

Figure 3

doi: https://doi.org/10.1371/journal.pone.0030894.g003