Molecular Cloning and Characterization of Two Genes Encoding Dihydroflavonol-4-Reductase from Populus trichocarpa
Figure 1
Nucleotide sequence analysis of PtrDFRs and their protein sequence alignments with other DFRs.
(A) A schematic representation of the exon and intron organization of PtrDFRs. PtrDFR1 consists of six exons (black boxes) and five introns (intervening line) with a 71-bp 5′ untranslated region (UTR; white box) and a 263-bp 3′ untranslated region. PtrDFR2 also consists of six exons (black boxes) and five introns (intervening line). (B) The amino acid sequence and structure of PtrDFRs. The boxed region represents a putative NADPH binding domain at the N terminus of PtrDFR proteins. GenBank accession numbers are as follows (in parentheses): PtrDFR1 (XM_002300723), PtrDFR2 (XM_002307631), PtDFR (AY147903), AtDFR (AAA32783), MtDFR1 (AY389346), MtDFR1 (AY389347) and OsDFR (BAA36182).