Caenorhabditis elegans Battling Starvation Stress: Low Levels of Ethanol Prolong Lifespan in L1 Larvae
Starved L1 larvae (100,000) were incubated in 100 ml of M9 only or 100 ml of M9 supplemented with 1 mM deuterated ethanol (C2D5OD) medium, under conditions described in Figure 1, and collected at six different time points. For each time point L1 larvae (100,000) were collected and total fatty acids analyzed after saponification and conversion to fatty acid methyl esters as described in the “Methods” section. Fatty acid methyl esters were fractionated and analyzed by GC-MS to look for the incorporation of deuterium atoms from the deuterated ethanol. Panel A shows the chromatogram for L1 larvae one day after hatching and before fully-deuterated ethanol was added. Panels C, E, G, I and K show the lipid profile for L1 larvae incubated in M9 medium for 1, 5, 7, 10 and 13 days, respectively. Panels D, F, H, J and L show the lipid profile for L1 larvae incubated in deuterated ethanol for 1, 5, 7, 10 and 13 days, respectively. Red arrows indicate the peaks containing deuterated fatty acids.