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Caenorhabditis elegans Battling Starvation Stress: Low Levels of Ethanol Prolong Lifespan in L1 Larvae

Figure 5

Intact pharyngeal pumping machinery is necessary for full ethanol-mediated lifespan extension of arrested wild-type L1 larvae.

Wild-type (N2) and eat-2(ad1116) mutant L1 larvae (70,000) were prepared as described in Figure 1, and were transferred to 70 ml of M9 medium only (wild-type (black line), eat-2 (gray line)) or 70 ml of M9 medium supplemented with 4 mM ethanol (wild-type (red line), eat-2 (blue line)) and survival at 20°C was scored every 48 hours until all larvae were dead. Mantel-Cox logrank analyses showed that lifespan was significantly different in the wild-type and eat-2 mutants in the presence of 4 mM ethanol (p<0.0001).

Figure 5

doi: https://doi.org/10.1371/journal.pone.0029984.g005