Antibodies against the Envelope Glycoprotein Promote Infectivity of Immature Dengue Virus Serotype 2
Figure 1
Effect of anti-E mAbs on the infectious properties of fully immature DENV particles.
P388D1 cells were infected with immature (prM-containing) DENV-2 strain 16681 at MOG 1000 in the presence or absence of: (A) anti-prM antibody and st DENV preparation without antibody, which were used as controls and (B–P) different anti-E mAbs. Prior to infection, immature DENV particles were incubated with varying antibody concentrations for 1 h at 37°C. At 43 hpi supernatant was harvested and virus production was analyzed by plaque assay on BHK21-15 cells. Data are expressed as means of at least two independent experiments performed in triplicate. Immature virus in the absence of antibodies was used as a control in all experiments (n = 20), for clarity the average of these results are only plotted in panel 1A. The error bars represent standard deviations (SD); (n.d.) denotes “not detectable”. Student's t-tests were used to determine significance; *, P<0.01.