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Persistence of Borrelia burgdorferi in Rhesus Macaques following Antibiotic Treatment of Disseminated Infection

Figure 6

Nucleic acid detection of B. burgdorferi (Experiment 2).

Detection by PCR or RT-PCR using primers for the indicated genes from: A) skin biopsy samples; B) xenodiagnostic ticks; C) organ tissue culture pellets; and D) directly from tissues known to harbor the spirochetes. Animal numbers in bold are of those that were treated. Asterisks indicate clear positive amplimers.sk = skin; h t = heart; bl = bladder; spl = pleen. Labels include: M = marker (100 bp ladder); (+) = B.burgdorferi, strain B31 DNA/RNA; ssc = spirochete strain negative control (Bb strain JD1 DNA); ntc = no template control; tnc = tick negative control; uim = uninfected monkey DNA/RNA.

Figure 6

doi: https://doi.org/10.1371/journal.pone.0029914.g006