Improved Culture-Based Isolation of Differentiating Endothelial Progenitor Cells from Mouse Bone Marrow Mononuclear Cells
Figure 6
FL cells improve cardiac functional recovery with reduced myocardial infarction.
DiI-acLDL labeled TT cells, AT cells or FL cells were injected to mice 3 days after induction of myocardial infarction, and the heart samples were harvested 3 days after cell injection. (A) The recruited DiI+ cells in ischemic border zone (left panels) and core zone (right panels) of myocardium were observed and counted under a fluorescent microscope. Dotted lines indicate the border of ischemic tissues and arrows indicate DiI+ cells. Each cell recruitment to ischemic myocardium was evaluated and expressed as a number of DiI+ cells in ischemic border zone (B) and in ischemic core zone (C) separately. *, P<0.05 vs. AT. Cardiac function was also evaluated by echocardiography 14 and 28 days after surgery. Increment in the parameter of fractional shortening (ΔFS) (D), left ventricular diameter at systole (ΔLVDs) (E), and left ventricular diameter at diastole (ΔLVDd) (F) were measured and averaged in the each cell-treated group. (n = 5) *, P<0.05 vs. AT. Mouse mature endothelial cells (MS-1) were used as another control.