Antibodies Recognizing Mycobacterium avium paratuberculosis Epitopes Cross-React with the Beta-Cell Antigen ZnT8 in Sardinian Type 1 Diabetic Patients
(A) Reactivity against the MAP-MBP fusion protein is MAP-specific. Ab+ and Ab-negative sera from T1D and healthy donors were challenged either with the MAP-MBP fusion protein (as in Fig. 1) or with a LacZ-MBP control protein. The dotted line indicates the cutoff for positivity. (B) Intra- and inter-assay variability of MAP3865c ELISA Ab assays. For intra-assay variability (white bars), the same serum was tested in 20 replicate wells; bars show readouts of each single well. CV is 2.8%. For inter-assay variability (grey bars), the same serum was tested in 4 separate experiments; bars show mean ± SEM of triplicate wells from each experiment. CV is 7.4%.