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Screening of Peptide Libraries against Protective Antigen of Bacillus anthracis in a Disposable Microfluidic Cartridge

Figure 7

Affinity Analysis of Single Clones.

Flow cytometry affinity analysis of the highest affinity clones for MMS and the best MACS/FACS clone. The cells were analyzed for affinity using 500, 250, 50, 25, 5, 2.5, 0.5, and 0 nM of the PA-Dylight 488 sample. The dissociation constant (KD) is shown for each sample.

Figure 7