Pseudomonas aeruginosa AES-1 Exhibits Increased Virulence Gene Expression during Chronic Infection of Cystic Fibrosis Lung
Figure 2
Genotyping of P. aeruginosa AES-1R, AES-1M and PAO1 by pulsed field gel electrophoresis.
Bacterial cells embedded in agarose plugs were lysed using EC lysis buffer (Sigma-Aldrich Australia), and digested using restriction enzyme SpeI to generate a small number (ca. 15–40) of large DNA fragments. Band patterns were analysed using cluster analysis software (GelComparII™, Applied Maths, Belgium) and established criteria [55] (same genotype if less than two bands difference). The AES-1R and AES-1M genomes fall within these criteria.