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Phosphorylation Alters the Interaction of the Arabidopsis Phosphotransfer Protein AHP1 with Its Sensor Kinase ETR1

Figure 4

Perrin plot analysis of recombinant ETR1, AHP1 and ETR1−AHP1 complex.

Purified proteins were dansylated and fluorescence anisotropy was recorded in 0.2 M potassium phosphate pH 8.0, 0.05% (w/v) β-D-dodecylmaltoside and 10 mM β-cyclodextrin at temperatures between 5°C and 25°C. Curves correspond to data obtained with 0.2 µM dansylated AHP1 (•), 0.2 µM dansylated ETR1 (○) and 2 µM ETR1 together with 0.2 µM dansylated AHP1 (▴).

Figure 4