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Phosphorylation Alters the Interaction of the Arabidopsis Phosphotransfer Protein AHP1 with Its Sensor Kinase ETR1

Figure 3

Perrin plot analysis of purified recombinant AHP1-GFP.

Measurements were performed in a buffer containing 50 mM Tris-HCl pH 7.5, 100 mM potassium chloride, 0.1% (w/v) β-D-dodecylmaltoside and 0.002% (w/v) phenylmethylsulfonyl fluoride at temperatures between 10°C and 30°C and glycerol concentrations of 10% (w/v) (Δ) and 20% (w/v) (▴). Closed circles (•) correspond to measurements at 0% (w/v) glycerol. At these conditions an increase in the molecular volume of the GFP-fusion protein is observed at temperatures below 17°C probably due to protein aggregation.

Figure 3