Isolation and Characterization of CvIV4: A Pain Inducing α- Scorpion Toxin
A. High performance liquid chromatography (HPLC) profile of C. vittatus venom fractions. Whole, soluble venom from C. vittatus was separated into five fractions (peaks: P1, P2, P3, P4, P5). Each fraction was isolated and tested for pain using the paw-licking assay in M. musculus. B. Mean (+1SE) duration of paw licking for M. musculus injected with water, scorpion venom, or venom fractions. Paw licking was recorded for five minutes following the injection. P4 was the only fraction that was significantly more painful than water (P<0.0001) and P4 induced as much pain as whole venom (P = 0.9525). Histograms showing the same letter did not differ at the P<0.05 level of significance using Tukey's HSD test.